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            In vitro pharmacodynamic function assessment of biological agents

            Target based Assay

            • Target validation

            • Binding and blocking assay


            1.  Protein based ELISA

            2.  Cell-based FACS

            3.  Dual binding of bispecific Ab (protein-protein, cell-protein, cell-cell)

            4.  ELISA/SPR, FACS 

            5.  RBC or platelets binding assay

            6.  Ligand blocking (protein or cell-based, ELISA/FACS)

            7.  Epitope binning (protein or cell-based, ELISA/FACS)

            8. Cross species/paralogue binding (protein or cell-based, ELISA, FACS)

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